Fig. 6

Transcriptomic analysis reveals significant mRNA expression changes in SiHa cells following HIV Tat and gp120 protein treatment. A Volcano plot displaying the differential expression of mRNAs. The vertical line represents a 1.5-fold change in expression (either upregulation or downregulation), while the horizontal line indicates the threshold for a 5% FDR. Red dots denote upregulated mRNAs, and yellow dots represent downregulated mRNAs. B Top 12 pathways associated with tumor biology functions, enriched with the differentially expressed mRNAs based on KEGG pathway analysis. The size of the bubbles indicates the number of enriched mRNAs, and the color reflects the p-value. The red frame highlights the Wnt signaling pathway, which is potentially linked to EMT. C Heatmap illustrating the expression pattern of key genes involved in the Wnt β-catenin pathway