Fig. 5
The transwell assay was employed to assess the effect of HIV gp120 protein and Tat protein on the invasiveness of SiHa cells. In the HIV gp120 + Tat protein treatment group, cells were exposed to HIV gp120 protein and Tat protein, both at a concentration of 100 ng/ml, for 5 days before being collected for the transwell assay. The transwell assay was conducted using a six-well transwell culture plate, with 1 × 105 cells seeded in 1 ml of cell culture medium per well. The figure displays representative images from three independent experiments. The accompanying bar chart depicts the number of cells that transmigrated through the membrane in SiHa cells with or without HIV-tat and gp120 protein treatment