Fig. 4
Changes in the wound healing rate of SiHa cells with/without HIV-tat and gp120 proteins treatment. SiHa cells were seeded in 6-well plates at a density of 1 × 105 cells per well. In the treatment group, cells were exposed to 100 ng/ml of HIV gp120 protein and 100 ng/ml of Tat protein. The culture medium, along with HIV-tat and/or gp120 proteins, was replaced daily. When cell confluence reached 90%, a uniform scratch was made using a 200 µl pipette tip to create a wound. Subsequently, images of the wound area were captured at 0 h, 24 h, and 48 h using a microscope. Image J software was employed to calculate the wound area. *P < 0.05. Representative images from three repeated experiments are presented. The scale bar is set at 400 μm