Inhibition of infection with pseudotyped HIV-1 in T cells andhuman astrocytes. (A) 1G5 T cells were treated with increasing concentrations of S. fusiforme and infected with either NL4-3 at 0.01 moi or with VSV/NL4-3 at 0.005 moi. 3 days after infection, % inhibition was calculated from luciferase expression from cell lysates adjusted to same number of viable cells by MTT. (B) Human fetal CD4 negative astrocytes were treated with 1 mg/ml S. fusiforme, or with 10-6 M ddC, infected with VSV/NL4-3 at 0.4 moi, and infection kinetics monitored by p24 expression in cell free supernatants at the indicated time points post infection. Data are mean +/- SD of triplicates. Representative of 2 experiments.