Inhibition of HIV-1 expression in human macrophages and microglia. Either, (A) human macrophages or (B) human fetal microglia were 24 h treated with 1 mg/ml S. fusiforme, or with 10-6 M ddC, infected with primary CCR5-tropic isolate ADA at 0.2 pg of p24/cell for 2 h, washed 3 times, and returned to culture with same concentration of each treatment for the duration of the experiment. At the indicated time points after infection HIV-1 expression was monitored by p24 production in cell-free supernatants by ELISA, % inhibition calculated as described in Methods and plotted on the y-axis. Data are mean +/- SD of triplicates. Representative of 2 experiments.