Time course of HIV-1 inhibition and viability in T cells. 1G5 T cells were 24 h treated with either 2 mg/ml S. fusiforme, or with 10-6 M ddC; then infected with NL4-3 at 0.01 moi for 1.5 h, washed 3 times, and returned to culture with same concentration of each treatment for the duration of the experiment. On day 3 post-infection, (A) gene expression of intracellular luciferase was measured from cell lysates adjusted to same number of viable cells, and % inhibition calculated and plotted on the Y-axis. Data are mean +/- SD of triplicates. In parallel, (B) cell viability was determined by trypan blue exclusion assay by counting at least 200 cells each, in three different fields under ×20 magnification using an Olympus BH-2 fluorescence microscope.